IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells
- VernacularTitle:肝癌细胞线粒体的纯化及双向凝胶电泳分析
- Author:
Xing LI
- Publication Type:Journal Article
- Keywords:
Mitochondria;
Density gradient centrifugation;
Two-dimensional gel electrophoresis
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To make the isolation and 2-DE analysis of mitochondria,in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation,and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable,which provides a basis for the following proteomic study on mitochondria.
