The impact of activin A neutralizing antibody on mRNA expression of cytokines in murine liver tissue
- VernacularTitle:激活素A中和抗体对小鼠肝组织细胞因子mRNA表达影响
- Author:
Na LI
;
Yingwei CHEN
;
Liangkai HU
- Publication Type:Journal Article
- Keywords:
Activin A;
Neutralizing antibody;
Hepatic fibrosis;
Cytokine
- From:
Chinese Journal of Digestion
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the effect of activin A neutralizing antibody (AANA) on inhibition of hepatic fibrosis and mRNA expression of various cytokines. Methods Mouse hepatic fibrosis model was induced by CCl_4. Male Kun-ming mice were divided into 5 groups: normal control group,olive oil control group, CCl_4 model group, AANA group and antibody control group. The mRNA expressions of activin A, transfer growth factor (TGF)-?_1, platelet derived growth factor (PDGF) and tissue inhibitors of metalloproteinas (TIMP)-1 in liver tissue were determined by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR) analysis. Results Compared with CCl_4 model group and antibody control group, the pathologic changes of hepatic fibrosis were ameliorated in AANA group, the mRNA expressions of activin A,TGF-?_1, PDGF and TIMP-1 were down-regulated in AANA group ( P
