Studies on Quality Standard of Wanbile Wan
- VernacularTitle:顽痹乐丸的质量标准研究
- Author:
Hualing XIA
;
Xinjie ZHAO
;
Guanghong HE
;
Juntao HAN
;
Zhiqian DU
- Publication Type:Journal Article
- Keywords:
Wanbile Wan;
Frutus Psoraleae;
Radix Puerariae;
Radix paeoniae A lba;
Radix Glycyrrhizae Preparate;
Isopsoralen;
Thin layer chromatography
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up the quality standard of Wanbile Wan. Method s Fructus Psoraleae, Radix Puerariae, Radix paeoniae Alba and Radix Glycyrrhiza e Preparatae in Wanbile Wan were identified by TLC .Isopsoralen was determined b y TLC scanning. The chromatographic conditions were: high performance silica gel G plate as lamellated plate, n- hexane - ethyl acetate (8 ∶ 2)being the exp ander, serrated scanning with single- wavelength reflection, beam stricture of 0.4? 0.4 mm, linear parameter SX=3 and scanning wavelength at 300 nm. Results The TLC spots were highly specific, clear and concentrated without interferen ce of the presence of negative controls. Isopsoralen showed a good linearity wit hin the range of 0.064～ 1.536 ? g, r=0.9980, average recovery rate was 100.7 % , and RSD was 2.0% .Conclusion This method can be used for the quality co ntrol of Wanbile Wan.
