Effect of Serum Containing Bushen Prescription on Chondrocyte Proliferation
- VernacularTitle:补肾方含药血清对软骨细胞增殖的影响
- Author:
Feng HUANG
;
Guoping XIE
- Publication Type:Journal Article
- Keywords:
BUSHEN PRESCRIPTION/pharmacology;
CHONDROCYTES/drug effects;
OSTEOARTHRITIS/TCD therapy;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To observe the effect of serum containing Bushen prescription (BP) on inhibition of chondrocyte proliferation and nitric oxide (NO) production in chondrocyte induced by interleukin-1 (IL-1). [Methods] Four New Zealand rabbits were administered BP 2.5mL (1.15kg/L) for 3 days for the preparation of BP-containing serum. Rabbit chondrocytes were cultured in-vitro and were added with DMEM culture containing IL-1 1, 10 and 20 ?g/L to examined the inhibition concentration of IL-1 for chondrocyte proliferation. After then, BP group was added with 20 ?g/L IL-1 and various concentrations of BP-containing serum and the model group with 20 ?g/L IL-1 and various concentrations of blank serum. Alter 3-day culture, chondrocyte proliferation were dectected by thiazolyl blue colorimetry (MTT) and NO content by Griess method. [Results] The inhibition concentration of IL-1 for chondrocyte proliferation was 20 ?g/L; various concentrations of BP-containing serum could counteract chondrocyte proliferation and inhibit NO pnxluction in chondrocyte induced by IL-1. [Conclusion] BP can counteract chondrocyte proliferation directly or indirectly by the mediation of NO, which may be one of its therapeutic mechanisms in treating osteoarthritis.
