Determination of Paeoniflorin Content in Kun'an Pills by HPLC
- VernacularTitle:高效液相色谱法测定坤安丸中芍药苷的含量
- Author:
Lifen ZHANG
- Publication Type:Journal Article
- Keywords:
KUN'AN PILLS/chemistry;
PAEONIFLORIN/analysis;
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To establish a method for the determination of paeoniflorin content in Kun'an Pills. [Methods] High pressure liquid chromatography (HPLC) was used. The chromatographic conditions were: Hypersil ODS column, acetonitrile-water (17 : 83) as mobile phase, detection wavelength at 230 nm and the flow rate being 1.0 mL/min. [Results] A good linearity of paeoniflorin was shown in the range of 0.096 8 - 0.484 0 ?g, r = 0.999 8; the average recovery was 97.84% and the relative standard deviation being 1.44%. [Conclusion] This method is simple and effective and can be used for the quality control for Kun'an Pills.
