Cloning, Sequencing and Homologous Analysis of Tyrosine Decarboxylase Gene from Aristolochia contorta Bge.
- VernacularTitle:马兜铃酪氨酸脱羧酶基因克隆、测序及同源性分析
- Author:
Ruiyi YANG
;
Qingping ZENG
- Publication Type:Journal Article
- Keywords:
ARISTOLOCHIA CONTORTA BGE. /chemistry;
TYROSINE DECARBOXYLASE;
GENES, STRUCTURAL, PLANT;
GENE AMPLIFICATION
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To obtain the tyrosine decarboxylase gene (tyrDC) from Aristolochia contorta Bge.and to assay its cDNA sequence and homologous analysis, thus to remove its nephrotoxieity. [Methods] The primers designed by referring to the conservative amino acid sequences of known plant tyrDC were used to amplify a fragment of cDNA from Aristolochia contorta Bge.by reverse transcription-polymerase chain reaction (RT-PCR). The amplified cDNA sequence was cloned and sequenced to design a pair of specific primers and to amplify a full-length tyrDC cDNA from Aristolochia contorta Bge.by rapid amplification of cDNA ends (RACE). [Results] The length of cloned tyrDC cDNA is 1678 base pairs (bp), which comprises an open reading frame ( ORF) of 1551 bp encoding 516 amino acids and a downstream untranslated region (3'UTR) of 127 bp. The results of sequence comparison indicated that the amino acid sequence deduced from the nucleotide sequence of tyrDC from Aristolochia contorta Bge.shares 76% homology with issued tyrosine decarboxylase of Papaver somniferum L. and 79% homology with tyrosine /DOPA decarboxylase from Thalictrum flavum subsp. glaucum. [Conclusion] The full-length tyrDC cDNA has been amplified from Aristolochia contorta Bge. and the homologous retrieve of tyrosine decarboxylase reveals an extensive sequence similarity among tyrosine decarboxylases of different plants. This will provide evidence for the romoval of nephrotoxicity of Aristolochia contorta Bge. .
