Significance of anti-cell membrane associated DNA (mDNA) antibodies in systemic lupus erythematosus
- VernacularTitle:抗细胞膜DNA抗体的测定及其在系统性红斑狼疮诊断中的意义
- Author:
Haiying CHEN
;
Rulin JIA
;
Zhanguo LI
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus, systemic;
Fluoroimmunoassay;
Cell membrane associated DNA antibody
- From:
Chinese Journal of Rheumatology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of anti-cell membrane associated DNA (mDNA) antibody detection, to evalute its sensitivity and specificity in systemic lupus erythematosus (SLE), and to analyze the relationship between anti-mDNA antibody and the clinical features of SLE. Methods Indirect immunofluorescence assay was used to measure anti-mDNA antibodies in sera of 207 SLE patients, 167 patients with other rheumatic diseases and 82 healthy controls. Using indirect immunofluorescence to detect the expression of mDNA with positive standard serum samples on nine cultured cell lines and pre-treated cells by DNAse, RNAse or trypsin. Results Of the serum samples, 73.3% SLE and 5.4% other rheumatic diseases were positive for anti-mDNA, but negative in 82 blood donors (P0.05). This study also proved that mDNA was expressed on B and T lymphocytes, the strongest staining was expressed on Raji cell line. The mDNA molecule was confirmed by pattern extinction on the cells pre-treated with DNAse but not RNAse or trypsin. Conclusion Anti-mDNA antibody is one of the most valuable marker in the diagnosis of SLE. Anti-mDNA antibody is valuable in diagnosis of SLE with negative anti-dsDNA, Sm, DNP, AHA and AnuA antibodies; but it has no significant with relationship the disease activity of SLE.
