Inhibition of tetramethylpyrazine-containing serum on proliferation of human liver cancer cells Hep G_2
- VernacularTitle:川芎嗪含药血清对人肝癌细胞HepG_2增殖的抑制作用
- Author:
Jundong FENG
;
Xiaoyu XU
- Publication Type:Journal Article
- Keywords:
tetramethylpyrazine (TMP);
human liver cancer cells Hep G2;
HPLC;
MTT assay
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of rat tetramethylpyrazine (TMP)-containing serum on the proliferation of human liver cancer cells Hep G2. Methods Thirty SD male rats were divided into three groups randomly and the serum was collected after ip TMP with the dosage of 143.0, 71.5 mg/kg or NS 0.8 mL to prepare the TMP-containing serum in large- and small-dose groups and NS group as well. A reversed-phase high performance liquid chromatography (RP-HPLC) method was used to determine the TMP concentration in rat serum. Human liver cancer cells Hep G2 was treated with rat TMP-containing serum for 48 h. Inhibition of the TMP-containing serum on proliferation of Hep G2 was detected by MTT assay. Results The serum of large dose TMP (20%, 10%, and 5%) and small dose TMP (20% and 10%) could obviously inhibit Hep G2 multiplication (P
