THE mRNA EXPRESSION OF CALCITONIN GENE RELATED PEPTIDE RECEPTORS AND THEIR ACTIVITY MODIFYING PROTEINS IN PERIAQUEDUCTAL GRAY OF RAT
- VernacularTitle:降钙素基因相关肽受体及其活性修饰蛋白mRNAs在大鼠中脑导水管周围灰质的表达
- Author:
Ning LI
;
Chunyan FANG
;
Er GAO
;
Fengbin WANG
;
Yuliang WANG
;
Guangxu ZHANG
- Publication Type:Journal Article
- Keywords:
Calcitonin gene related peptide;
Calcitonin gene related peptide receptor;
Receptor activity-modifying proteins;
Periaqueductal gray;
RT-PCR;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In the present study we used real-time quantitative RT-PCR to measure the relative expression levels of CGRP,CRLR,RDC-1,RAMP1-3 in periaqueductal gray(PAG)of intact rats. Methods With ?-actin as an endogenous control,write down the C T value and calculate the relative amount of every sample against its endogenous control. Results The results showed the specific amplification of CGRP,CRLR,RAMP1-2 in PAG of rats.Furthermore,the expression of CGRP and RAMP1 is the highest,that of CRLR is higher and the RAMP2 is low;whereas no band is detected in RDC-1 and RAMP3. Conclusion These findings suggest the specific expression of CGRP,CRLR,RAMP1 and RAMP2 mRNA and indicate their functional role in the modulation in PAG of intact rats.
