EFFECT OF ROSIGLITAZONE, A LIGAND OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA, ON THE BIOLOGICAL CHARACTERS OF HEPATIC STELLATE CELLS
- VernacularTitle:过氧化物酶体增殖物活化的受体?特异配体罗格列酮对肝星状细胞生物学特性的影响
- Author:
Yantong GUO
;
Xisheng LENG
;
Tao LI
;
Shenghan SONG
;
Zhizhong QIN
;
Liangfa XIONG
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator-activated receptor gamma(PPAR?);
Ligand;
Rosiglitazone;
Hepatic stellate cells(HSC);
RT-PCR;
Western blot;
Immunocytochemistry;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of rosiglitazone, a ligand of peroxisome proliferator-activated receptor gamma (PPAR?), on the expression of PPAR? in hepatic stellate cells (HSCs) and its effect on the biological characters of HSCs. Methods The activated HSCs were devided into three groups:control, 3??mol/L rosiglitazone group, and 10??mol/L rosiglitazone group. The expression of PPAR?, ?-smooth muscle actin(?-SMA), and type Ⅰ and Ⅲ collagen was detected by means of RT-PCR, Western blot and immunocytochemistry respectively. The cell proliferation was determined with MTT colorimetric assay. The cell apoptosis was demonstrated with flow cytometry. Results The expression of PPAR? at mRNA and protein level markedly increased in HSCs of 10??mol/L rosiglitazone group(t≥4.627, P
