Research of the mechanism of pre-B-cell colony-enhancing factor in pulmonary vascular endothelial permeability increasing after CPB
10.3969/j.issn.1671-8348.2013.28.021
- VernacularTitle:前B细胞克隆增强因子在体外循环术后肺血管内皮通透性增加中的机制研究
- Author:
Yuan ZENG
;
Wei YANG
;
Xiaoqiang ZHANG
;
Bin LI
;
Xiao DONG
- Publication Type:Journal Article
- Keywords:
PBEF;
cardiopulmonory bypass;
pulmonary vascular endothelial;
permeability
- From:
Chongqing Medicine
2013;(28):3388-3389,3392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of PBEF in pulmonary vascular endothelial permeability increasing after car-diopulmonory bypass ,in order to provide the basis for a better lung protective measures during cardiopulmonary bypass .Methods Animal models were established ,group A :the rats were transfected with the lentiviral AD-PBEFshRNA ;group B :the rats were took 30 min deep hypothermic circulatory arrest ;group C :the rats were took 30 min deep hypothermic circulatory arrest ,then trans-fected with the lentiviral AD-PBEFshRNA ;control group :the rats were anesthetized and established CPB tube ,without CPB by-pass .Lung tissue was detected with Western blotting and ELISA .Results PBEF ,phosphorylation of P38MAPK ,ERK ,MLC ,VE-cadherin ,FAK in group C had significant difference with group A ,B and the control group(P<0 .05) .VEGF ,MMP2 ,MMP9 ,W/D in group C had significant difference with group A ,B and the control group(P<0 .05) .The pathological results showed that rat lung tissue of the control group was normal ,while in group C ,it had severe pathological damage ,the pathological damage degree in group A ,B reduced compared to group C .Conclusion PBEF can through MAPK/PI3K-Akt/VEGF signaling pathway ,increase pulmonary vascular endothelial permeability .
