The effect of tumor necrosis factor receptor-p55/p75 on pathogenesis of severe acute pancreatitis in rats
- VernacularTitle:肿瘤坏死因子受体p55/p75在重症急性胰腺炎发病机制中的作用
- Author:
Qi ZHU
;
Weiyan YAO
;
Yaozong YUAN
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Tumor necrosis factor-?;
Tumor necrosis factor receptor-p55;
Tumor necrosis factor receptor-p75
- From:
Chinese Journal of Digestion
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tumor necrosis factor receptor-p55/p75(TNFR-p55/ TNFR-p75) on the pathogenesis of severe acute pancreatitis (SAP) in rats. Methods Thirty-six male Sprague-Dawley rats were divided into 2 groups: SAP group and control group. SAP model was ~induced by injection of 5% sterile sodium taurocholate into biliopancreatic duct, while control group was only given sham operation. Rats were sacrificed at 3, 6, and 12 hours after the onset of operation. Blood sample and pancreatic tissues were collected. The severity of pancreatitis was assessed according to the level of serum amylase and histological scoring. The serum levels of tumor necrosis factor-?(TNF-?) were examined by ELISA. Expressions of TNFR-p55 mRNA and ~TNFR-p75 mRNA in pancreatic tissues and peripheral blood mononuclear cells (PBMC) were measured by semi-quantitative RT-PCR. Results The levels of serum amylase and TNF-? in SAP group were both significantly higher than those in the control group at each time point (P
