The optimal cryopreservation liquid of the stable transfected cell line named hepatic carcinoma HepG 2 Transfected by Lintivirus
10.3969/j.issn.1671-8348.2013.28.023
- VernacularTitle:不同冻存液冻存慢病毒转染后稳转肝癌细胞株 HepG2的效果比较
- Author:
Jia LI
;
Huiping LU
;
Zhenbo FENG
;
Weijia MO
- Publication Type:Journal Article
- Keywords:
transfection by lentivirus;
hepatic neoplasms;
cell cryopreservation
- From:
Chongqing Medicine
2013;(28):3393-3394,3397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the optimal cryopreservation liquid of the stable transfected cell line named hepatic carcino-ma HepG2 transfected by lentivirus .Methods Glycerol and DMSO as cryopreservation liguid ,The HepG2 transfected by lentivirus and HepG2 cells were both cryopreserved with four various proportion of cryopreservation liquid .After thawing ,the survival rate of the two cells were observed by inverted contrast microscope and the viability and proliferation were detected with MTT assay .Re-sults Using glycerol as cryoprotectant liquid ,the survival rate of the HepG2 cells transfected by lentivirus was obviously higher than other 3 groups when the proportion of the cryopreservation liquid(DMEM ∶FBS∶Glycerol)was 0∶9∶1(P=0 .001) ,where-as there was no significant difference among the groups of HepG2 cells with various proportion of cryopreservation liquid (P=0 .293) .Using DMSO as cryoprotectant liquid ,the survival rate of the cells transfected by lentivirus was 0% ,whereas the survival rate of the HepG2 cells was higher than 60% ,and there was no significant difference between groups of various proportion of cryo-preservation liquid(P=0 .487) .The MTT assay demonstrated that the higher the serum levels was ,the better proliferation capacity those cells had(P<0 .05) .Conclusion The optimal cryopreservation liquid for the hepatic carcinoma cell line HepG2 transfected by lentivirus is used glycerol as cryoprotectant solution ,and the proportion of cryopreservation solution was 90% FBS + 10%Glycerol .
