The Regulating effects of interleukin-1? on gene expression of metalloproteinase-13 mRNA in human hyaline chondrocytes
- VernacularTitle:白细胞介素-1?对人软骨细胞基质金属蛋白酶-13 mRNA表达的作用
- Author:
Jianhong QI
;
Qinghua ZHAO
;
Yanju LIU
;
Yalu LI
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinase;
Interleukin-1;
Cell culture;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Rheumatology
2002;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of interleukin-1? (IL-1?) in regulating the gene expression of matrix metalloproteinase-13 (MMP-13) in the human hyaline chondrocyte. Methods Human hyaline chondrocytes were harvested enzymatically and cultured in DMEM supplemented with 20% fetal bovine serum, varying concentration of IL-1? was added to the medium. The MMP-13 mRNA level of passaged monolayer cell cultures of human hyaline chondrocyte was assessed by reverse transcriptase polymerase chain reaction (RT-PCR) and fluorescent quantitative PCR (FQ-PCR). Chondrocyte cDNA was amplified byGeneAmp 5700 sequence detector system with equal quantitative genomic cDNA as templates respectively. Results IL-1? could increase the MMP-13 mRNA level in passaged cultures of hyaline chondrocyte. Their difference was significant (P
