HPLC fingerprints of Herba Artemisiae scopariae
- VernacularTitle:茵陈的HPLC指纹图谱研究
- Author:
Xiangyu DENG
;
Guoxiang SUN
- Publication Type:Journal Article
- Keywords:
high performance liquid chromatography;
fingerprint;
Herba Artemisiae scopareiae;
chromatographic fingerprint index;
chromatographic fingerprint relative index
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: A novel method for the overall quality control of Herba Artemisiae scopareiae has been established by HPLC fingerprint. METHODS: The chromatographic fingerprints were obtained by injecting ~5 ?L of the sample solution each time on a Kromasil ODS column (250 mm?~4.6 mm ,~5 ?m ) with the gradient elution solvent system composed of 1%acetate acid-water-methanol. The flow rate was 1 mL/min, the column temperature was maintained at ~30 ℃ and the detection wavelength was set at ~326 nm . RESULTS:22 co-possessing peaks were selected as the fingerprint peaks of Herba Artemisiae scopareiae and the similarities between the chromatographic fingerprints of the herbal drugs were calculated taking chlorogenic acid peak as the reference peak. The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index (F), the relative index (Fr) and the fingerprint components apparent prercentage contents (P%). CONCLUSION: This method with good precision and reproducibility can be useful in the quality control of Herba Artemisiae scopareiae.
