Determination of tanshinone Ⅱ_A and cryptotanshinone in Compound Danshen Tablet by RP-HPLC
- VernacularTitle:RP-HPLC测定复方丹参片中丹参酮Ⅱ_A和隐丹参酮的含量
- Author:
Renmin LIU
;
Aixia DENG
;
Daojie LIU
- Publication Type:Journal Article
- Keywords:
reversed-phase high performance chromatography;
Compound Danshen Tablet;
tanshinone Ⅱ_A;
crytatoshinone
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To set up a determination of tanshinone Ⅱ_A and cryptotanshinone in Compound Danshen Tablet(Radix Salviae Miltiorrhizae, Radix Notoginseng, Borneolum Syntheticum) by RP-HPLC. METHODS:Two components could be separated through YWG-C_~18 column with methanol-water (75∶25 by volume) as a mobile phase. The flow rate was 1.0 mL?min~-1 , and the detection wavelength was at 270 nm. RESULTS: The linear range of tanshinone Ⅱ_A and cryptotanshinone were 2.5-50.0 ?g?mL~-1 and 0.625-12.5 ?g?mL~-1 , respectively. The average recovery was 99.76% with RSD 0.92% for tanshinone Ⅱ_A and 98.34% with RSD 0.71% for cryptotanshinone, respectively. CONCLUSION: The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of Compound Danshen Tablet.
