Determination of hirudo in Zhiyu Granules by anti-thrombin bioactivity
- VernacularTitle:抗凝血酶效价法测定蛭萸颗粒剂中的水蛭成分
- Author:
Ping MAO
;
Wanjing WU
;
Xu XU
;
Guizhen YU
- Publication Type:Journal Article
- Keywords:
Zhiyu Granule;
hirudo;
qualitative;
anti-thrombin bioactivity
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the qualitative method for determining hirudo in Zhiyu Granule (Scorpio, Scolopendra, Rhizoma Coptidis, Radix Astragali, etc.) METHODS: Hirudo was determined by anti-thrombin bioactivity method after being extracted from Zhiyu Granule using 95% ethanol. RESULTS: The difference between Zhiyu Granule and the control sample without hirude was significant and easy to identify. CONCLUSION: After being extracted by 95% ethanol, hirudo can be identified from other anti-thrombin bioactivity components in prescription.