Determination of paeoniflorin in mouse plasma after ig compound decoction of Radix Paeoniae Rubra with Fructus Piperis by RP-HPLC
- VernacularTitle:RP-HPLC法测定灌胃赤芍胡椒复方小鼠血浆中芍药苷的含量
- Author:
Jin PEI
;
Zuyi YANG
;
Rongmin LIU
;
Jia CHENG
;
Deguang WAN
;
Ron HU
- Publication Type:Journal Article
- Keywords:
compound decoction of Raidx Paeoniae Rubra with Fructus Piperis;
paeoniflorin;
HPLC;
blood concentration
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective An HPLC method was established for determination of paeoniflorin in plasma after ig compound decoction of Radix Paeoniae Rubra with Fructus Piperis to mice. Methods The conditions of chromatography: Kromasil C 18 column (250 mm ? 4.6 mm, 7 ?m) was used with a mobile phase of CH 3OH-H 2O ( 38∶ 62); flow rate was 0.5 mL/min; the detecting wavelength was 230 nm; external standard method was quantitative analysis method. Results Paeoniflorin was fully separated from the other components in plasma. The linear range was 5.0—250.0 ng/?L (r= 0.999 9 ), the lowest detectability was 1.49 ng/?L, the average recovery was higher than 90%. Conclusion This method specially provides an accurate and sensitive way in detecting the in vivo blood concentration of paeoniflorin in plasma.
