Preparation of Polyporus umbellatus polysaccharides long circulating liposomes
- VernacularTitle:猪苓多糖长循环脂质体的制备
- Author:
Kaiping WANG
;
Yu ZHANG
;
Jun ZHANG
- Publication Type:Journal Article
- Keywords:
Polyporus umbellatus polysaccharides (PUPS);
long circulating liposomes (LCLs);
encapsulation efficiency;
UV-Sephadex method
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the Polyporus umbellatus polysaccharides (PUPS) long cirrculating liposomes (LCLs) and to study the quality control of PUPS LCLs. Methods The PUPS LCLs were prepared by chloroform infusion combined with the ammonium sulphate gradient method. The content and encapsulation efficiency of PUPS in LCLs were determined by UV-Sephadex method. Results Mean diameter of the PUPS LCLs was 100 nm, with the encapsulation efficiency of 55.3%. Conclusion The LCLs with high encapsulation efficiency and small particle size could be prepared by chloroform infusion combined with the ammonium sulphate gradient method. UV-Sephadex method is suitable for the quality control of PUPS LCLs and the results are reliable.
