Application of ISSR-PCR to identification of different Dendrobium Sw. species
- VernacularTitle:ISSR-PCR在石斛种间鉴别中的应用
- Author:
Ying SHEN
;
Cheng XU
;
Xiaofeng WAN
;
Ming ZHANG
- Publication Type:Journal Article
- Keywords:
inter-simple sequence repeats-polymerase chain reaction (ISSR-PCR);
molecular marker;
Dendrobium Sw.;
identification
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify nine species of Dendrobium Sw. by inter-simple sequence repeats-polymerase chain reaction (ISSR-PCR) method and to investigate the difference among the species of Dendrobium Sw. at DNA molecular level. Methods Ten primers constituted by simple sequence repeats (SSR) were tested for PCR and sepharose electrophoresis of the nine species. Results Seven of the ten primers amplified polymorphic bands. The number of polymorphic band positions of each primer ranged from 7 to 14, and the size of fragments ranged from 220 to 1 260 bp. The amplification patterns of two primers, namely UBC-807 and UBC-864, were higher in terms of polymorphic and amplified band ratio. Each of them was able to distinguish all the examined species. Conclusion ISSR-PCR provides a quick, reliable molecular marker technique for identification of different species of Dendrobium Sw.
