In - Vitro Experiment for Detecting Microdialysis Recovery rate of Sinomenine
- VernacularTitle:青藤碱微透析回收率测定方法的体外模拟实验
- Author:
Jiajun LING
;
Rui LI
;
Liling ZHOU
- Publication Type:Journal Article
- Keywords:
SINOMENINE/chemistry;
MICRODIALYSIS PROBE;
CHROMATOGRAPHY, HIGH PRESSURE LIQUID;
SINOMENINE / pharmacokinetics
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To explore the in-vitro determination method for the recovery rate of sinomenine in microdialysis probe and to investigate the influencing factors. [Methods] The recovery rate of sinomenine was detected by concentration difference method (by gain and by loss) and zero-net flux method. [Results] The recovery rate detected by increment method was as the same as that by decrement method; the recovery rate had no correlation with the sinomenine concentration in the solvent. Sinomenine recovery assessed by concentration difference method had a good intra-day stability and intra-day reproducibility. Sinomenine concentration and recovery in the solvent could be determined accurately by zero-net flux method. [ Conclusion ] Microdialysis sampling can be used for the pharmacokinetic study of sinomenine and decrement method (ie. retrodialysis) can be used for the determination of recovery rate of sinomenine.
