Observation of the preparation and morphology of the small intestinal submucosa sponge
10.3760/cma.j.issn.1008-6706.2013.21.001
- VernacularTitle:猪小肠黏膜下层海绵的制备和形态学观察
- Author:
Wei TIAN
;
Xiaojie WANG
- Publication Type:Journal Article
- Keywords:
Small intestinal submucosa sponge;
Morphological observation
- From:
Chinese Journal of Primary Medicine and Pharmacy
2013;20(21):3201-3203,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective The sponge of the small intestinal submucosa(SSIS) is established by the material chemistry,in order to supply the database for the scaffold of the dermic tissue engineering.Methods At the regular temperature,the SSIS was conducted firstly by the physiochemical method.Then,the SSIS was crosslinked by the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) so as to get the three dimentional SSIS.The morphological feature was obtained by the lighting and electron microscope,ophthalmic oberservation,the embedding in the mouse of the SSIS was used to observe the adsorbing and immunology.The results were analyzed by statistic software.Results The crosslinked SSIS was regular in the structure and not loosen,the framework was equilibrium,the pore was abundant with the diameter of the 100 ～ 200μm,the difference was obvious (all P ＜ 0.05).The firstweek after embedding did not exist the adherence leukocyte infiltration,the vascularization occur in the third week.The material was completed and had no elastic degradation.Conclusion Reconstructed SSIS has completed structure,elastic with the vascularization and minor immunology,which could be used as the extracellular scaffold for the dermic tissue engineering.
