Studies on HPLC Fingerprint of Triterpene Acids in Poria Peel
- VernacularTitle:茯苓皮中三萜酸类成分HPLC指纹图谱的方法学研究
- Author:
Shaojun WANG
;
Qi DUAN
;
Shilin YANG
;
Qianfeng GONG
;
Jihua ZHANG
- Publication Type:Journal Article
- Keywords:
Poria peels;
Triterpene acids;
HPLC;
Fingerprint
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for HPLC fingerprint determination of the triterpene acids in poria peel.Methods Ethanol solvent and ultrasound method were used to extract the sample. The chromatographic conditions of RP-HPLC were:acefonitrile-0.5%phosphoric acid(linear gradient elution),the detection wavelength being 242 nm, temperature at 30℃and flow rate being 1.0mL?min-1. Ten batches of poria peel produced in different places were measured.The common model was set up to calculate the correlation coefficient, cos?between every batch vector and the total standard model vector, thus to define the similarity.Results There was much difference in the constituent content of poria peel produced in different places and batches.Conlusion The HPLC fingerprint of triterpene acids is stable and reprodacible,which is useful for standard cultivation and quality evaluation of the crud drug of poria peel.
