Determination of Shikonin in Radix Arnebiae Oil by HPLC
- VernacularTitle:HPLC法测定紫草油中左旋紫草素的含量
- Author:
Yi WU
- Publication Type:Journal Article
- Keywords:
Radix Arnebiae Oil;
Shikonin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the content determination of shikonin in Radix Arnebiae Oil by HPLC.Methods The content of shikonin was determined by HPLC and the chromatographic conditions were: Shim-pack VP-ODS column(4.6 mm?250 mm),the mobile phase consisting of methanol-0.025 mol/L H3PO4(85 ∶15),detection wavelength being 516 nm,tempeature at 25 ℃and the flow rate being 20 ?L.Results A good linearity of shikonin was in the range of 11.2 ?g/mL～33.6 ?g/mL(r=0.9998),and the average recovery rate was 101.03 %,RSD=1.91%.Conclusion This method was simple,accurate and with a good reproducibility and can be used for the quality control of Radix Amebiae Oil.
