The effect of hydrocortisone on epithelial apoptosis in fetal mice intestine
- VernacularTitle:氢化可的松对小鼠胚胎肠上皮凋亡的影响
- Author:
Haiying ZHANG
- Publication Type:Journal Article
- Keywords:
Methylmercury chloride;
Fetal mouse;
Intestinal epithelium;
Apoptosis;
Protein expression
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of hydrocortisone (HC) on apoptosis and expression of apoptosis- associated proteins in the intestinal epithelium of fetal mice.Methods:The duodena and colons were taken from E13.5d and E14.5d fetal mice respectively,and cultured with different doses of HC (0 ?g/ml,0.5?g/ml,1.0?g/ml,2.0?g/ml).After 24h,the samples were sliced up into the paraffin sections.Some sections were stained with HE to count the numbers of apoptotic bodies (NAB)in the fetal intestinal epithelium with stereological method.Others were stained with the immunohistochemical method to observe the expression of Bcl-2,Bax,P53 and c-Fos.Results:①In each experimental group,the NAB were higher than that of control group( P
