Preparation of immunoliposomes encapsulating alkaline phosphatase
- VernacularTitle:包裹碱性磷酸酶免疫脂质体的制备
- Author:
Jinjiang HUANG
- Publication Type:Journal Article
- Keywords:
Alkaline phosphatase;
Immunoliposome;
Antibody;
Preparation
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare immunoliposomes encapsulating alkaline phosphatase and to evaluate the methods.Methods:The liposomes containing alkaline phosphatase were prepared by reverse phase evaporation or film dispersion.Liposomes and rabbit anti-human IgG were conjugated by glutaraldhyde or deriving antibody.Not only did the liposomes encapsulate alkaline phosphatase but also they had immune activity.All methods were analyzed and compared.Results:Many liposomes prepared by reverse phase evaporation had perfect shape and high bioactivity of alkaline phosphatase.The glutaraldhyde method conjugating antibody was convenient,efficient and practical.Conclusion:Immunoliposomes with bioactivity of enzyme and antibody are prepared through reasonable methods.
