Determination of baicalin, rhein and berberine in Yiqing Capsule by HPLC
- VernacularTitle:HPLC同时测定一清胶囊中黄芩苷、大黄酸和小檗碱的含量
- Author:
Yi LI
;
Xu XU
- Publication Type:Journal Article
- Keywords:
HPLC;
Yiqing Capsule;
baicalin;
rhein;
berberine
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the contents of baicalin, berberine, rhein in Yiqing Capsule(Radix et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis) by HPLC. METHODS: The HPLC system consisted of Kromasil C 18 column (150 mm?4.6 mm, 5 ?m), 0.02 mol?L -1 phosphoric acid (TEA adjusted to pH=6.82)-acetonitrile(40∶11) mixture as mobile phase, detection wavelength at 254 nm, flow rate of 1.0 mL?min -1. RESULTS: The calibration curves of three ingredients were linear within the range of 3.666-73.32 mg?L -1(r=0.9998, baicalin), 0.3112-15.56 mg?L -1(r=0.9998, rhein), 1.221-50.04 mg?L -1(r=0.9999,berberine), respectively. The average recovery of baicalin is 102.3%(RSD=2.5%), berberine for 101.8%(RSD=1.8%), and rhein for 98.3%(RSD=2.9%). CONCLUSIONS: The method is rapid, sensitive and accurate.
