Effect of exercise on GLUT4 in adipocyte of rats with insulin resistance
- VernacularTitle:运动对胰岛素抵抗大鼠脂肪细胞葡萄糖转运蛋白-4的影响
- Author:
Juanjuan LI
;
Huimin BI
;
Feng LI
;
Baohua WANG
- Publication Type:Journal Article
- Keywords:
Exercise;
GLUT4;
Insulin resistance;
Adipocyte
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of exercise on glucose transporter 4 (GLUT4) in adipocyte of rats with insulin resistance induced by diets. Methods Experimental rats were randomized into three groups: a control group (n=10), a model group (n=10) and an exercise group (n=10). The model and exercise groups were fed with the diets enriched with sucrose (20%,w/w), lard (10%, w/w), cholesterol (2.5%, w/w) and cholic acid (1%, w/w) to induce insulin resistance. Exercise group was subject to swim for 6 weeks. After the plasma membrane and the intracellular membrane of adipocyte were prepared and separated, GLUT4 protein was detected by Western blot analysis. The body weight, serum triglyceride and cholesterol, fasting plasma glucose and serum insulin concentration were detected at the predetermined time points. And the insulin sensitivity index was calculated. Results Western blot analysis showed that intracellular membrane GLUT4 protein in adipocyte of the rats in the model group decreased by 38.72% (P
