Effect of Fertilization Promoting Peptide on Kinematic Parameters, Capacitaion and Acrosome Reaction in Human Spermatozoa.
- Author:
Moon Kyoo KIM
;
Hee Gyoo KANG
;
Myo Kyung KIM
;
Dong Hoon KIM
;
Sung Won HAN
;
Do Hyun CHOI
;
Ho Hoon LEE
- Publication Type:In Vitro ; Original Article
- MeSH:
Acrosome Reaction*;
Acrosome*;
Chlortetracycline;
Exocytosis;
Fertilization*;
Fluorescence;
Humans*;
Male;
Semen;
Spermatozoa*
- From:Korean Journal of Fertility and Sterility
2000;27(2):201-208
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: This study has been carried out to evaluation the effect of fertilization promoting peptide (FPP) on the kinematic parameters, capacitation and acrosome reaction of the frozen-thawed human spermatozoa. METHODS: After FPP treatment, we examined kinematic parameters, capacitation and acrosome reaction, using the methods of computer-aided sperm analysis (CASA) and chlortetracycline (CTC) fluorescence analysis. RESULTS: We have obtained the evidence that FPP can promote the capacitation and inhibit the spontaneous acrosome reaction of frozen-thawed human spermatozoa in vitro. Fpp (25~100 nM) induced a significant increase in the proportion of B-pattern capacitated spermatozoa, and a significant decrease in the proportion of F-pattern uncapacitated ones without significant stimulation of acrosomal exocytosis. In the kinematic parameters treatment, FPP treated groups maintained higher LIN, BCF and STR than those of control. The VAP, VSL, VCL and ALH were not different. Therefore it is suggested that FPP in human seminal plasma may play a positive role in promoting human sperm function.
