The protective effect N-acetyl-L-cysteine against reperfusion injury on donor rat lung
- VernacularTitle:N-乙酰-L-半胱氨酸对供体肺的保护作用
- Author:
Dong WANG
;
Qingyu WU
;
Xingpeng CHEN
- Publication Type:Journal Article
- Keywords:
Lung Organ preservation solutions Reperfusion injury Nuclear factor kappa B Cell adhesion mulecules
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect by inhibiting nuclear factor-kappa B (NF-?B) by N-acetyl-L-cysteine(NAC) on donor rat lung. Methods 24 SD rats were randomly divided into two groups, the control group and the experiment group. The harvested lung blocks were flushed with and stored in the low-potassium-dextran (LPD) solution in control group, but in the experiment group, LPD solution containing NAC was used. The lungs were preserved at 4℃ for 16 hours in both groups. Isolated rat lung reperfusion models were established and the donor lungs were perfused for 1 hour. PaO_2 and PAwP were measured at every 15 min intervals during reperfusion. After reperfusion, the lung tissue wet-to-dry (W/D) ratio and myeloperoxidase(MPO) activity were obtained. The protein and mRNA expressions of intercellular adhesion molecule-1(ICAM-1) and nuclear factor-kappa B(NF-?B) were also observed by using immunohistochemistry and semi-quantitative RT-PCR at the end of reperfusion. Results After reperfusion for 60 minutes, in experiment group, PaO_2 drop in and increase PAwP lower than in control group (P
