Tissue culture of medicinal plant Swertia mileensis
- VernacularTitle:药用植物青叶胆的组织培养
- Author:
Hengyu HUANG
- Publication Type:Journal Article
- Keywords:
Swertia mileensis T.N.Ho et W.L.Shih;
tissue culture;
callus;
adventitious bud
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to preserve the natural resources of Swertia mileensis which has been destroyed seriously, the method of artificial propagation by way of tissue culture has been systematically studied. Methods The immature stems and the mature leaves were the best material explants in rapid propagation in all of the experiments. These explants were cultured on MS culture media by adding different portions of hormones at various cultural conditions. Results For the leaves, the suitable phytohormone combination to induce callus are Zt 0.5 mg/L+NAA 0.1 mg/L+IBA 0.1 mg/L or BA 0.5 mg/L+2,4-D 0.1 mg/L+IBA 0.1 mg/L; for the stems, they are BA 0.02 mg/L+Kt 0.04 mg/L+IBA 0.05 mg/L; for the adventitious buds, they are BA 2.0 mg/L+NAA 0.5 mg/L or BA 2.0 mg/L+IBA 0.1 mg/L; and for the roots, they are MS+Kt 0.01 mg/L+IBA 0.5 mg/L+NAA 1.0 mg/L. Conclusion Tissue culture of S. mileensis can make its propagation rapid, its resources preserved, and its utilization last.
