EXPERIMENTAL STUDY ON THE RECONSTRUCTION OF THE NERVE-MUSCLE STRUCTURE AND FUNCTIONAL RECOVERY OF THE SCIATIC NERVE GAP BY ACELLULAR ALLOGRAFTS IN RAT
- VernacularTitle:脱细胞异体神经移植物桥接大鼠坐骨神经缺损促进神经肌结构重建和功能恢复的实验研究
- Author:
Xiaojie TONG
;
Caishun ZHANG
;
Deshou CAO
;
Chengji LIU
;
Pin YU
- Publication Type:Journal Article
- Keywords:
Peripheral nerve regeneration;
Motor end-plate reconstruction;
Motor functional recovery;
Silver combined AChE;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The experiment was designed to investigate the effect of acellular nerve allografts on the functional recovery and reconstruction of the nerve-muscle structure of the sciatic nerve defect in rats. Methods Acellular nerve allograft was transferred into the defected rat sciatic nerve with 10mm long.The wet weight of tibialis anterior was weighed at 12 and 24 weeks postoperatively compared with control group.The conducted velocity of regenerated nerve and the effect of regenerated nerve on tibialis anterior were investigated by electrophysiologic test,and silver staining combined with AChE histochemical methods were used in the experiment separately. Results The wet weight of tibialis anterior and the conducted velocity of regenerated nerve in experimental group were similar to those in control group in 12 and 24 weeks after transplantation.The positive acetylcholinesterase(AChE)histochemical reaction was observed in the tibialis anterior at 12 weeks with deeper staining and located in the middle of tibialis anterior tidily at 24 weeks after operation.The regenerated nerve bundles and nerve terminals were found to grow into the motor end-plate of the tibialis anterior in silver staining combined with AChE staining in experiment group.Electromyogram showed that the regenerated nerve has innervated tibialis anterior already.Conclusion The results indicated that extracted nerve allografts as a bridge can promote the motor functional recovery and reconstruction of the nerve-muscle structure of the defected rat sciatic nerve.
