THE EXPRESSION OF NF-?B IN SPINAL GANGLIA OF THE ASTHMATIC GUINEA PIGS AND THE INHIBITORY EFFECT OF DEXAMETHSONE
- VernacularTitle:NF-?B在哮喘豚鼠脊神经节的表达及地塞米松的抑制作用
- Author:
Junhao YAN
;
Zhenyu WANG
;
Xiaoxiang LIU
;
Xiubi FANG
- Publication Type:Journal Article
- Keywords:
Nuclear factor-?B(NF-?B);
Bronchial asthma;
Spinal ganglia;
Dexamethsone;
Immunohistochemisitry;
In situ hybridijation;
Guinea pig
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of NF-?B in C-7-T-5 spinal ganglia and possible mechanism of treating asthma with dexamethsone. Methods The alterations of NF-?B activity and its mRNA expression were investigated by means of immunohistochemistry and in situ hybridization histochemistry combined with the micro-image analysis in C-7-T-5 spinal ganglia in the asthmatic and dexamethsone-treated guinea pigs. Results The NF-?B immunoreactivity was found mainly in cytoplasm and weakly in nucleus,its mRNA was expressed weakly in C-7-T-5 spinal ganglia in the control groups.The NF-?B immunoreactivity was more in nucleus and less in cytoplasm,the expression of its mRNA increased significantly in C-7-T-5 spinal ganglia in the asthmatic group compared with the control groups(P0.05).Conclusion The present results indicate that NF-?B in C-7-T-5 spinal ganglia might be involved in the pathogenesis of the bronchial asthma,and inhibiting the expression and activity of NF-?B in C-7-T-5 spinal ganglia might be one of the mechanisms of treating the bronchial asthma with dexamethsone.
