Assay of rubimaillin in Shenqiangujing Granules
- VernacularTitle:参茜固经颗粒中大叶茜草素含量测定
- Author:
Yuwei TANG
;
Binxian SUN
;
Dejie HUANG
- Publication Type:Journal Article
- Keywords:
Shenqiangujing Granules;
rubimaillin;
HPLC;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC for the determination of rubimaillin in Shenqiangujing Granules (Radix Codonopsis, Radix Rubiae, Radix Rehmanniae, Fructus Ligustri Lucidi, etc.) METHODS: HPLC column was C-18 (250 mm?4.6 mm, 5 ?m). The mobile phase was methanol-water-tetrahydrofuran (310∶90∶3). Flow rate was 1.0 mL?min -1 . The detection wavelength was at 249 nm. RESULTS: The linear range of rubinaillin was in the range of 0.056～0.29 ?g, r=0.9999. The average recovery was 99.36% (RSD=1.60%, n=5). CONCLUSION: The method is accurate with the good reproducibility, and can be used for the determination of rubimaillin in Shenqiangujing Granules.
