Determination of puerarin and baicalin in Gegen Qinlian Micropill by HPLC
- VernacularTitle:HPLC双波长法测定葛根芩连微丸中葛根素、黄芩苷的含量
- Author:
Kewei ZHANG
;
Hao CAI
;
Yumei CHI
;
Xiaobing CUI
;
Wei LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Gegen Qinlian Micropill;
dual wavelength;
puerarin;
baicalin;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC for determination of puerarin and baicalin in Gegen Qinlian Micropill. METHODS: A Kromasil C 18 Column was used. The mobile phase was methanol-0.2% phosphoric acid. Puerarin and baicalin were determined by dual wavelength, ? 1=250nm. ? 2=315nm. RESULTS: The linear range of puerarin was within 32.0 ng—288.0 ng,r=0.9998 and the sample recovery was 100.17 % (RSD=1.00%(n=9)). The linear range of baicalin was within 52.6 ng～447.1 ng, r=0.9999 and the sample recovery was 100.06 % (RSD=1.06% (n=9)). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control of Gegen Qinlian Micropill.
