Sulfation techniques of Bletilla striata polysaccharide by orthogonal design
- VernacularTitle:正交试验优选白芨多糖硫酸酯化工艺的研究
- Author:
Jingyao CHEN
- Publication Type:Journal Article
- Keywords:
Bletilla striata (Thunb.) Reichb. f. polysaccharide (BSPS);
sulfation;
orthogonal design
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the sulfation-conditions of Bletilla striata polysaccharide (BSPS). Methods BSPS was sulfated with chlorsulfonic acid-pyridine. Reagent proportion, reaction temperature, and reaction time were selected with substitution degree and yield as index by L_9(3~4) orthogonal design. Results Results showed that the condition with 6∶1 volume ratio of pyridine and chlorine sulfonic acid, reaction temperature of 85 ℃, reaction time of 2 h was the optimum. Conclusion The optimal method for sulfation of BSPS is feasible, and it provides valuable parameters for further enlarged test.
