Analysis of genetic diversity in certified Radix Gentianae by RAPD and ISSR
- VernacularTitle:正品龙胆遗传多样性的RAPD及ISSR分析
- Author:
Yanan CAO
;
Qingzhang LI
;
Yue SUN
;
Lu LI
;
Dawei WANG
;
Jingpeng LI
- Publication Type:Journal Article
- Keywords:
RAPD;
ISSR;
certified Radix Gentianae;
genetic diversity
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the sibling relationship and genetic diversity of four certified Radix Gentianae (RG) species by RAPD and ISSR methods. It provided molecular biological proof for species identification and breeding of certified RG. Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data. Results Ten RAPD primers and four ISSR primers were selected respectively from 100 RAPD primers and 32 ISSR primers. The statistic data were analyzed to construct cluster analysis dendrogram by genetic distance UPGMA method. Conclusion The PCR reaction systems of this experiment gets ideal RAPD and ISSR results that suit to the analysis of certified RG species in genetic diversity and sibling relationship.
