In-Vitro Culture of Different Explants from Andrographis paniculate (Burm. f.) Nees
- VernacularTitle:穿心莲不同外植体离体培养的研究
- Author:
Hong HE
;
Changjie HUANG
;
Changhui LIU
;
Yuru LI
;
Honghua XU
- Publication Type:Journal Article
- Keywords:
ANDROGRAPHIS PANICULATA (BURM. F.) NEES/growth & development;
MEDICINAL HERB ANIMAL RAISING
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
[ Objective ] To supply a technological evidence for the rapid reproduction of Andrographis paniculata (Burm. f.) Nees (APN). [Methods] Different explants from sterile seedling of Andrographis paniculata (Burm. f.) Nees were cultured in vitro to induce the production of fascicular buds. [ Results ] Cotyledon with nodes and half cotyledon with nodes were the most suitable explants for culture, and the budding rate was 100% and over 90% respectively. 6-Benzylaminopurine (BA) in the concentrations of 0.5 ~ 1.0mg/L were beneficial for the reproduction and growth of buds. The age of explants had no correlation with budding. The optimal rooting medium was MT (Murashige and Tucher) medium added with Img/L NAA (naphthaleneacetic acid) or IBA (indolebutyric acid). A highest rooting rate as much as 61.9% was achieved after 10 days of culture. [Conclusion] An effective plant regeneration system has been established for explants of Andrographis paniculata (Burm. f.) Nees.
