Elementary Studies on HPLC Fingerprint of Yuanshi Shengmai Chenggu Tablets
- VernacularTitle:袁氏生脉成骨片HPLC指纹图谱的初步研究
- Author:
Yuechun HUANG
;
Ping XI
;
Hongmei TANG
;
Huifang ZENG
- Publication Type:Journal Article
- Keywords:
Yuanshi Shengmai Chenggu Tablets;
HPLC;
Fingerprint
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the fingerprint of Yuanshi Shengmai Chenggu (YSC)Tablets by HPLC.Methods Chromatographic conditions of HPLC were as follows: Hypersil ODS2 column with temperature at 20 ℃; methanol and 1 %acetic acid glacial(gradient elution)as a mobile phase; detection wavelength at 268 nm;analytical time being 55 min and flowing rate being 1.0 mL/min.Results Twenty-five peaks were indicated on the HPLC-fingerprint of YSC Tablets. The peak area of vitexin was the biggest area in different batches of YSC Tablets, the area being 22 %~25 %. The differences of fingerprint in different batches of YSC Tablets were not obvious, which indicated the fingerprint characteristics of YSC Tablets.Conclusion The method is simple and accurate and with a good reproducibility and can be used for the quality control of YSC Tablets.