Determination of Cinnamic Acid in Cortex Cinnamomi by RP-HPLC
- VernacularTitle:高效液相色谱法测定肉桂不同部位中肉桂酸的含量
- Author:
Qin FANG
;
Jiyin XU
;
Ping DING
;
Honghua XU
- Publication Type:Journal Article
- Keywords:
Cortex Cinnamomi;
Cinnamic acid;
RP-HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the RP-HPLC method for the determination of cinnamic acid in different parts of Cortex Cinnamomi which comes from Wulong and Xinyi in Guangdong province. Methods The samples were assayed on a DiamonsilTM C18 column(250 mm?4.6 mm,5 ?m)with temperature at 40℃. The mobile phase was aqueous acetonitrile-0.01 %phosphoric acid (gradient elution); flow rate was 1 mL/min and the detection wavelength was at 278 nm. Results The contents of cinnamic acid in one-year branches were the highest in the samples from the two differents habitats.Conclusion The method is simple,reliable,repeatable,and is suitable for the determination of cinnamic acid in Cortex Cinnamomi.
