Determination of Calycosin-7-?-O-D-glucopyranpside in Danggui Buxue Dropping Pills by HPLC
- VernacularTitle:高效液相色谱法测定当归补血滴丸中毛蕊异黄酮苷的含量
- Author:
Jianping SONG
;
Hua XIE
- Publication Type:Journal Article
- Keywords:
Danggui Buxue dropping pills;
Calycosin-7-?-O-D-glucopyranpside;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of calycosin-7-?-O-D-glucopyranpside in Danggui Buxie Dropping Pills.Methods HPLC method was used. The chromatographic column was YMC-C18(150?4.6 mm, 5 ?m), the mobile phase was methanol and water(40 ∶60), the detection wavelenth was 235 nm, column temperature at 20 ℃and flow rate was 1.0mL/min.Results Calycosin-7-?-O-D-glucopyranpside was separated well with other components and was in good linearity in the range of 0.02～0.80 ?g. The average recovery was 97.62 %and RSD=1.06 %.Conclusion The method is rapid and accurate and can be used for quality control of Danggui Buxie Dropping Pills.
