Expression of Toll like receptor 4 and MD-2 gene and protein in Kupffer cellsfollowing ischemia-reperfusion injury in rat liver transplant
- VernacularTitle:大鼠肝移植缺血再灌注后Kupffer细胞Toll样受体4及其伴侣分子MD-2的表达
- Author:
Yong PENG
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Ischemia-reperfusion injury;
Toll like receptor 4;
Kupffer cell
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of Toll like receptor 4(TLR4) and MD-2 gene and protein in Kupffer cells and its role in ischemia-reperfusion injury (IRI) on rat liver graft.Methods:The Kupffer cells were isolated at 0(control group),2,12,24h (IR group)following IR.The mRNA and protein expressions of TLR4 and MD-2 were detected by RT-PCR and flow cytometry (FCM),respectively.The TNF-? level in supernatant was measured by ELISA.Then the isolated KCs were incubated with anti-TLR4 polyclonal antibody (anti-TLR4 group),and the TNF-? level were measured again.Results: The mRNA and protein expressions of TLR4/MD-2 and the level of TNF-? in IR group increased significantly following IR compared with those in control group( P
