Study on the effct of MMP-13 and its tissue inhibitors,metalloproteinase-1 on the rat of COPD model
- VernacularTitle:MMP-13与TIMP-1表达变化及其在COPD大鼠肺结构改建中的作用
- Author:
Tao CHEN
- Publication Type:Journal Article
- Keywords:
Chronic obstructive pulmonary disease;
Rat;
Collagenases;
Tissue inhibitor of metalloproteinase-1;
Remodel
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of the collagenases MMP-13 and its inhibitor, tissue inhibitor metalloproteinase-1 (TIMP-1) in the rat lung of the experimental models of COPD.Methods:Male Wistar rats (10 weeks of age) were divided into two groups—model and control groups.The rat of model group were given intratracheal lipopolysaccharide (LPS),and then animals were exposed to cigarette smoke for 32 days.The lung function was measured,and pathological changes were also observed.Transcriptional levels of MMP-13 and TIMP-1 mRNA extracted from the lungs were assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).Results:(1)In COPD model group,the pathological changes of bronchi and lung tissue,the changes of lung function were similar to those of the COPD patients.(2)The mRNA expression of MMP-13 and TIMP-1 in COPD model group were significantly increased compared with those in control group( P
