RNA interfering on the cell cycle of gastric cancer by the recombinantplasmid affecting DNMT1 gene expression
- VernacularTitle:DNMT1靶向RNAi重组体对胃癌细胞周期的影响
- Author:
Daorong CHEN
- Publication Type:Journal Article
- Keywords:
DNMT1;
RNA interfering;
Gastric cancer;
Cell cycle
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone the recombinant plasmid affecting gene DNMT1 expression by RNA interfering and transfect it into AGS in order to further search its effects on the cell cycle of gastric cancer AGS.Methods:Two DNA sequences containing short hairpin structure was designed and synthesized.The complement form was obtained by annealing and inserted into vector pTZU6+1.The recombinant plasmid was transfected into AGS.Finally the cell cycle was evaluated through flow cytometer.Results:The S cycle cells of the transfected AGS dropped dramatically.And the G2-M cycle cells increased significantly.Conclusion:Successful cloning and transfecting the recombinant make it possible to search new gene therapy for the tumors.
