Analysis of drug resistance and genotype of extended-spectrum?-lactamase producing Enterobacteriaceae bacteria
- VernacularTitle:肠杆菌科产超广谱?-内酰胺酶菌株的耐药性及基因型分析
- Author:
Biao XU
- Publication Type:Journal Article
- Keywords:
Enterobacteriaceae;
Extended-spectrum ?-lactamase;
Drug resistance;
Genotype
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the drug resistance and genotypes of extended-spectrum ?-lactamase(ESBL)-producing Enterobacteriaceae bacteria in our hospital.Methods:257 strains of Enterobacteriaceae bacteria were isolated from clinical specimens in our hospital from Sept.2002 to Oct.2003.Sixty-three strains of ESBLs-producing isolates were detected by phenotypic confirmatory test according to NCCLS M100-S9.The susceptibility test of them to the antibiotics were detected with Bio-Merieux ATB G-5.The SHV-type,TEM-type,CTX-M-type encoding genes were amplified by PCR.Results:The resistant rates of the 63 strains to IMP,MERO,CXT,FEP,CAZ,AKN,CTX and CIP were 1.6%,1.6%,38.1%,39.7%,46.0%,55.6%,63.5% and 95.2%,repectively.The rates of Enterobacteriaceae bacteria carrying SHV-type,TEM-type,CTX-M-type ESBLs were 84.1%,47.6% and 25.4%,repectively.Conclusion:Multidrug resistance of the ESBLs-producing strains in our hospital is obvious.IMP and MERO are still the best choices for treatment of the infections caused by ESBLs-producing bacteria.Most of the ESBLs-producing strains carry SHV-type.CTX-M-type ?-lactamase exists prevalently in our hospital.
