Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection
- VernacularTitle:紫外吸收碘量法快速测定体液维生素C
- Author:
Xiaoyun ZHU
- Publication Type:Journal Article
- Keywords:
Vitamin C;
Iodometry;
UV absorbance detection
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of fast assay of vitamin C (VC) in body fluids by iodometry with UV absorbance detection.Methods:The sample was pretreated with iodoacetic acid to block sulfhydryl group.Protein was precipitated by trichloroacetic acid,and chromogen was extracted with chloromethylene.The change of absorbance after 30 s reaction of the pretreated sample with iodine was measured.Results:The assay of absorbance before and after 30 s reaction of the pretreated sample with iodine showed the variation coefficient within 5%,and the recovery of VC was above 90%.There was linear response of the absorbance change to the amount of VC from body fluids with slope consistent to that for iodine.Conclusion:This method may be desirable for fast assay of VC in body fluids.
