PARTIALY INHIBITION OF THE EFFECTS OF ESTRODIOL ON RETINAL ENDOTHELIAL CELL BY NGF SIGNALING BLOCKAGE
- VernacularTitle:阻断神经生长因子信号部分抑制雌二醇对恒河猴视网膜血管内皮细胞的作用
- Author:
Baoyang HU
;
Kun LIU
;
Jin WAN
;
Honghua GONG
;
Zhenjue SHE
;
Honglei XIAO
;
Guomin ZHOU
- Publication Type:Journal Article
- Keywords:
Estrogen;
Nerve growth factor;
Retinal;
Vascular endothelial cells;
Tubogenesis;
Rhesus
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the estradiolNGF regulatory cascade in retinal endothelial cells. Methods Retinachoroids vascular endothelial cell line RF/6A from rhesus was cultured in M199 medium contain 20% FBS.mRNA of ER,NGF and its receptors,TrkA and P75 NTR were detected with RTPCR.The cells were incubated with NGF,VEGF,antibodies against NGF or VEGF,and K252a(100nmol/L),the specific inhibitor of TrkA,separately or in different combination.MTT based cell counting assay was used to study the viability of the cells.The apoptosis was evaluated by FACS,mass migration by wound healing assay,and tubogenesis by AngioMatrix assay. Results We amplified the specific fragments of cDNA of ER,NGF and NGF receptor TrkA using RTPCR.10?nmol/L1??mol/L estradiol augments the proliferation and increases the viability of RF/6A in a dosage dependent manner.In the wound healing based migration assay,we found the similar alteration.This effects of estradiol was partially blocked by NGF neutralized antibody and K252a.Apoptosis rates were at the similar level among the groups.For the tubogenesis of RF/6A,we found no augmentation by NGF,and no blocked augmentation by estradiol.Conclusion NGF,first identified as the survival factor of nerve system,now also seemed to be an activator of retinal endothelial cell,is under the regulation of estrogen.The proliferation and migration,but not the tubogenesis of retinal endothelial cells are regulated by this regulatory cascade.
