Relationship of hypoxia-inducible factor-1 ? expression and vascular endothelial growth factor in SKOV-3 ovarian cancer model
- VernacularTitle:卵巢上皮性癌组织中缺氧诱导因子1?的表达及其与血管内皮生长因子的相关性
- Author:
Hongyuan JIANG
;
Youji FENG
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Carcinoma;
Transcripti on factors;
Vascular endothelial growth factor A
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of hypoxia-inducible factor (HIF)-1? and va scular endothelial growth factor (VEGF) or micro-vessel density ( MVD). Methods The ovarian cancer cell line SKOV3 was transplanted into nude mice to form xenog eneic tumor. Mice were treated with rapamycin 4mg/kg,sulindac 100 mg/(kg.d) a nd saline 200 ?l respectively. Expression of HIF-1? and VEGF proteins and MV D were determined by immunohistochemistry. The mRNA of Glut1 and VEGF was studi ed by RT-PCR. Results The positive expression of HIF-1? and VEGF was moderate to strong, and MVD was high (31?8) in control group. In rapamycin treated group, the expression of H IF-1? was inhibited to weak positive or negative (P
