Effects of endothelin-1 on proliferation and changes of Ca~(2+) in activat ed hepatic stellate cells
- VernacularTitle:内皮素-1对活化肝星状细胞增殖及钙离子影响的调控机制研究
- Author:
Xixian YAO
;
Dongmei YAO
;
Hongmei FANG
- Publication Type:Journal Article
- Keywords:
Endothelin-1;
Hepatic stellate cells;
Ca 2+;
Extra-regulated kinase
- From:
Chinese Journal of Digestion
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the effects of endothelin-1(ET-1) on expression of extra-regulated kinase 1(ERK 1), cell cycle and changes of Ca 2+ in activated hepatic stellate cells (HSCs). Methods Effects of different concentra tions of ET-1 on ERK 1 expression were determined by Western blotting. Effects of ET- 1 on cell cycle were observed by flow cytometry(FCM) analysis. Effects of ET-1 on changes of calcium concentration and cell area were observed by laser scanning confocal microscopy (LSCM). Results ET-1 could inhibit the expression of ERK 1 i n a dose-dependent manner, compared with the control, the expression of ERK 1 in E T-1 highest concentration group decreased 10.91%?3.36% (P
