Inhibitory Effect of Kaixin Capsule on Gene Expression of Myocardial Immune Inflammatory Factor in Dogs After Resuscitation
- VernacularTitle:开心胶囊对复苏犬心肌细胞免疫炎症因子基因表达的抑制作用
- Author:
Xiaoxing LUO
;
Dawei WANG
;
Yonghua ZHAO
;
Jian LIANG
- Publication Type:Journal Article
- Keywords:
KAIXIN CAPSULE/therapeutic use;
CARDIOPULMONARY RESUSCITATION;
CYTOKINES;
DISEASE MODELS, ANIMAL;
DOGS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of Kaixin Capsule (KC) on gene expression of myocardial immune inflammatory factor in dogs after resuscitation and to search for an effective way to counteract the progress of systemic inflammatory response syndrome (SIRS) after cardiopulmonary resuscitation. 【Methods】 Fifteen hybrid dogs were randomly divided into three groups, 5 dogs each: pseudo-operation group (group A), model group (group B) and KC group (group C). The dog model of cardiac arrest and resuscitation was established; the dogs were executed and the heart was isolated 180 min after the recovery of autonomic circulation. Myocardial total RNA was extracted and the mRNA expression of objective gene was detected by reverse transcription polymerase chain reaction (RT-PCR). 【Results】 mRNA expression of Toll-like receptor (TLR_4) and tumor necrosis factor ?(TNF-?) in group B differed from that ingroup A (P
